dermal cell basal medium plus melanocyte growth kit  (ATCC)

Journal: Febs Letters

Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation

doi: 10.1002/1873-3468.70195

Figure Lengend Snippet: Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

Article Snippet: A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Irradiation

Journal: ACS Nano

Article Title: Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker

doi: 10.1021/acsnano.5c01873

Figure Lengend Snippet: Altered organization of filamentous actin and microtubule cytoskeletons in melanoma cancer cells. Maximum intensity Z-projections (XY) demonstrating alterations in filamentous actin (magenta) and microtubule cytoskeletons (green). Immunofluorescence images of cells were captured using a Zeiss LSM 900 Airyscan 2. Compared with benign counterpart fibroblasts and melanocytes, highly malignant melanoma cells exhibit significant decreases in F-actin and microtubule network density.

Article Snippet: Human primary epidermal melanocyte cells were obtained from ATCC (Cat. # PCS-200-013) and cultured in Dermal Cell Basal Medium (ATCC, Cat. # PCS-200-030) supplemented with Phenol Red (ATCC) and Adult Melanocyte Growth Kit (ATCC, Cat. # PCS-200-042).

Techniques: Immunofluorescence